编码小鼠Chst4的基因(在BAC克隆RP23-362O14中)与包含cre重组酶cDNA的PCR片段通过同源重组方式结合,旁边是50bp的Chst4序列,这些序列环绕着kanamycin抗性基因,该基因被frt座标记。线性化的重组BAC被注入了受精卵,这些卵子被移植到伪怀孕的代孕母体内。产生了四个杂交瘤系,其中一条被用于后续研究。(来源:J:147714)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x C3H/HeN)F1
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4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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