将一个荧光素酶cDNA与第2外显子非编码区连接,形成一个带有内切的诺姆素表达载体,该载体包含一个合成的poly(A)序列来终止转录。这个构建被插入到第2外显子下游,上游增加了一个loxP位点。通过Cre重组酶介导的第2外显子和诺姆素元件的切除后,荧光素酶将由内源性启动子控制表达。(来源:J:147139)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/SvImJ
Targeted
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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