构建了一个转基因,其中经过修改的人类血管内皮生长因子1(COMP-Ang1)cDNA(由CAG启动子通过loxP-bleasticidineS-DNA-polyA-loxP序列分隔)与之相连。这个构建被注入到TT2胚胎干细胞中。随后,将表达Cre的质粒导入了转染的ES细胞中,筛选出两个克隆显示了COMP-Ang1高表达。来自克隆5的转基因小鼠由此产生。当与合适的Cre表达线杂交时,这些后代将表达改良的人类血管内皮生长因子1形式。(来源:J:147388)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
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插入
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1
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5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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