将编码第1至117个氨基酸的首个外显子替换为pgk-抗氨苄青霉素抗性 cassette,通过同源重组实现。 Western blot结果显示,该剪切蛋白得到了表达。(来源:J:147050)
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The first coding exon, encoding exons 1 through 117, was replaced with a pgk-neomycin resistance cassette via homologous recombination. Western blot analysis confirmed the expression of the truncated protein. (J:147050)