将编码Hcst和Tyrobp的所有外显子替换为新插入的neo cassette。通过骨髓衍生巨噬细胞和IL-2激活的杀伤细胞提取物的 Western blot 分析,证实了蛋白表达的缺失。(来源:147155)
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All the exons that code for Hcst and Tyrobp were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on bone marrow derived macrophage and IL2-activated killer cell extracts. (J:147155)