通过同源重组,将一个cre/ERT2-IRES-EGFP-frt的neo cassette插入到5'非翻译区。之后,通过Flp介导的重组技术去除了neo cassette。cre/ERT2转录物和EGFP蛋白的表达模式与内源性蛋白一致。(来源:J:147282)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count