Exon 1被替换成新霉素抗性基因座。PCR检测在纯合小鼠中未发现任何mRNA,而 Western blot分析在胸腺细胞和脾细胞中也未检测到任何蛋白质。(来源:J:145839)
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Exon 1 was replaced with a neomycin selection cassette. PCR analysis could not detect any mRNA in homozygous mice and Western blot analysis did not detect any protein in either thymocytes or splenocytes. (J:145839)