Exon 3周围有loxP位点,下游插入了含FRT标记的诺卡因抗性基因座。在将胚胎囊胚微注射前,通过短暂表达Flp重组酶来剔除诺卡因基因座。随后,这些小鼠与EIIa-cre转基因小鼠杂交,以去除含loxP标记的等位基因。对纯合胚胎成纤维细胞提取物进行免疫印迹分析未检测到蛋白质。(来源:J:146616)
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cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count