Exon 3周围有loxP位点,下游插入了含FRT标记的诺卡因抗性基因座。在将胚胎囊胚微注射前,通过短暂表达Flp重组酶来剔除诺卡因基因座。随后,这些小鼠与EIIa-cre转基因小鼠杂交,以去除含loxP标记的等位基因。对纯合胚胎成纤维细胞提取物进行免疫印迹分析未检测到蛋白质。(来源:J:146616)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
基因内删除
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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