Azin1^{Gt(Ayu17-689)Imeg}

The gene trap vector, pU-17, integrated at a single site in the mutant cell line. This vector is composed of a mouse En2 splicing acceptor, a lox71 site, beta galactosidase, neomycin, loxP, a poly A site, and lox2272. The integrated trapping vector was found to have a 522 bp deletion at its 5' terminus. Vector insertion was in intron 2 about 9.295 kb upstream of exon3 in which the translational start codon is located. Northern blot analysis demonstrated that gene expression was inhibited in heterozygotes and strongly suppressed in homozygotes. (J:146570)