整个编码区域通过同源重组被新插入的neo cassette替换。 Western blot分析证实了纯合小鼠肌肉中未检测到可察觉的蛋白表达。(来源:J:146419)
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The entire coding region was replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of detectable protein expression in muscles from homozygous mice. (J:146419)