Tg(CSNK1D*,-EGFP)827Yfu

The human BAC clone RP11-1376P16 containing the CSNK1D gene was targeted to generate a threonine-to-alanine alteration at amino acid 44 in the protein (T44A). An IRES-GFP cassette was also inserted after the stop codon of the CSNK1D gene. Six lines were generated with two of the lines, 827 and 859, being high expressers due to insertion of 2 to 3 copies of the transgene. Transgene expression was confirmed by RT-PCR of brain lysates. This is a representative record for the two high expressing lines. (J:97658)