Cert1^{Gt(RRF047)Byg}

The gene trap vector, pGT2Lxf, was inserted between exons 3 and 4 disrupting the pleckstrin homology domain and resulting in a truncated protein product. The exact site of insertion was 18394 nucleotides downstream of the 5' end of the third intron. RT-PCR analysis failed to detect full length transcript in homozygous embryos. Western blot analysis detected little or no protein expression in homozygous embryos. (J:146172)