基因拼接修复被用来复制exon 2,并在两个exon 2拷贝之间插入了一个neo cassette,通过脑组织的 Western blot 分析证实了蛋白表达缺失。(来源:J:145944)
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Gene gap repair was used to duplicate exon 2 and inserted a neo cassette between the two copies of exon 2. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:145944)