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中文
使用了一种带有自剪切LoxP位点和S-mu开关区上游的neomycin/sperm特异性Cre cassette的靶向载体，目标对准了LoxP位点和位于E-mu增强子下游的I-SceI内切酶位点。该构建通过电穿孔技术导入了C57BL/6的白化胚胎干细胞（ES）细胞。经过正确筛选的ES克隆被注入受体囊胚中。产生的雄性后代能够自行剪切掉neomycin/Cre cassette，保留LoxP和I-SceI位点在生殖细胞中。通过Southern blotting确认了精确的靶向。杂合子小鼠的B细胞发育和数量正常。（来源：J:145691）
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
