Tg(Scgb1a1-rtTA,tetO-VEGFA)1Eli

The CC10-rtTA-hGH construct contains the Scgb1a1 promoter, the reverse tetracycline transactivation (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-VEGF-hGH construct contains a polymeric tetracycline operator (tet-O), minimal cytomegalovirus (CMV) promoter, cDNA for the human 165 amino acid isoform of VEGF-A and hGH intronic and polyadenylation signals. Both constructs were coinjected into fertilized oocytes and dual positive founder animals were obtained. In the presence of doxycycline (dox), rtTA is able to bind in trans to the tet-O, and human VEGF-A is expressed. In the absence of dox, rtTA binding occurs at very low levels, and only low level gene transcription is noted. (J:93253)