Hprt1^{tm14(Ple103-EGFP/cre)Ems}

The Ple103-EGFP/cre transgene (pEMS1083) was designed with the 877 bp Ple103 minipromoter (HAP1-A; derived from a segment of the basal promoter element of the human huntingtin-associated protein 1 (HAP1) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtsb-m3 mutant locus on the X chromosome. (J:144244, J:164356)