germ line(生殖细胞)、由Cre介导的重组被用来去除cDNA和exon 3。蛋白表达的缺失通过在小鼠胚胎成纤维细胞中进行 Western blot 分析得到了确认。(来源:J:145315)
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Germ line, cre mediated recombination was used to remove the cDNA and exon 3. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblasts. (J:145315)