基因的11到16外显子,包括55%的编码区域,通过同源重组被新插入的neo cassette替换。 Western blot分析证实了纯合小鼠肝脏中没有蛋白表达(来源:J:144968)
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Exons 11 - 16, encompassing 55% of the coding region of the gene, were replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in livers from homozygous mice. (J:144968)