选择标记和转录终止子被-loxP位点包夹,并被置于一个融合基因上游。这个融合基因编码Tcfe2a(E47)的转录激活区域和Tal1的bHLH域。下游放置了一个IRE-SGFP cassette,整个构建体-loxP-neo-STOP-loxp-Tcfe2a/Tal1-IRE-GFP被敲入到定位点。当Cre重组酶表达时,融合蛋白和GFP会被表达,预测融合蛋白由于对E因子的强亲和力,对Id和Tal蛋白家族成员有拮抗性作用。(来源:J:144569)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129X1/SvJ
Targeted
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3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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