Lyn^mld4

ENU mutagenesis of mice carrying the Mpltm1Wsa allele caused a C-to-A mutation in the 25th nucleotide pair of exon 12. This mutation is predicted to cause the substitution of a lysine for a threonine at residue 429 in the highly conserved Src activation loop of the protein product. Founder mice were bred with wild-type mice to breed away the Mpltm1Wsa allele. Mutant protein isolated from the B cells of homozygous mice was found to lack kinase activity in an in vitro transkinase assay. (J:144799)