从2号外显子到7号外显子的一个基因片段被替换了新插入的neo cassette。通过在E13.5整个胚胎提取物中进行 Western blot 分析,确认了没有蛋白质表达(来源:J:142392)。
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A genomic fragment spanning from part of exon 2 through exon 7 was replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on E13.5 whole embryo extracts. (J:142392)