Gnas^tm3Kel

The Nesp transcript lies within the Gnas locus and is normally subject to maternal imprinting so that only the paternal allele is expressed in offspring. The Nesp transcript was truncated by replacing the splice donor site of the second Nesp exon with a cassette comprising the final exon, polyadenylation, and downstream sequences of the rabbit beta-globin gene and a floxed neomycin selection cassette. Chimeric founders were crossed with cre-deletor mice to excise the selection cassette. Heterozygotes were maintained by paternal transmission of the allele. Nesp transcript was not detected in the brain of neonatal heterozygotes that had maternal transmission of the mutant allele. (J:143477)