整个SbcC域的序列通过同源重组被PGK-neo cassette替换。 Western blot结果显示,纯合小鼠睾丸中未检测到该蛋白。(来源:J:144218)
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The entire sequence encoding the SbcC domain was replaced with a PGK-neo cassette via homologous recombination. Western blot analysis confirmed the absnece of protein in testes from homozygous mice. (J:144218)