在翻译起始点上游66bp的第2外显子的EcoRI位点插入了cre/ERT2载体,同时伴随一个由Frt序列引导的PGK-neomycin筛选 cassette。随后,这些杂合子小鼠与Tg(ACTFLPe)9205Dym背景的鼠进行杂交,以剔除neomycin筛选 cassette。(来源:J:144088)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count