从2号外显子到5号外显子被替换为lacZ基因和一个floxed neo cassette。通过脑和脊髓提取物的 Western blot 分析,证实了没有蛋白质表达(来源:J:142504)
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Exons 2 through 5 were replaced with a lacZ gene and floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on brain and spinal cord extracts. (J:142504)