Apc^tm1Tyj

A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. (J:159014)