所有3个外显子都被通过同源重组替换为neo cassette。 Western blot分析证实了在纯合小鼠的肺、脾、胸腺和心脏中没有检测到蛋白(来源:J:142966)
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All 3 exons were replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein in the lung, spleen, thymus, and heart of homozygous mice. (J:142966)