The TRP-1 TCR was generated from immunized Tyrp1B-w mice. Clone 7A6 expressed Valpha3.2Vbeta14 TCR chains. These TCR sequences were cloned into separate vectors and co-injected into microinjected into C57BL/6 fertilized eggs. Founder line 9 was subsequently established and the transgene insertion site was found on the Y chromosome. (J:138466)