从2号到4号外显子通过小鼠的生殖细胞进行了基因敲除,通过Cre介导的重组。蛋白表达缺失通过肾、肝和心脏提取物的 Western blot 实验得到了确认(来源:J:142586)
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Exons 2 through 4 were removed by germ line, cre mediated recombination. The absence of protein expression was confirmed by western blot analysis on kidney, liver and heart extracts. (J:142586)