Tg(Cyp21a1-Apoe)619Fet

A construct containing approximately 6 kb of the mouse Cyp21a1 promoter attached to the mouse Apoe gene was used for injection into fertilized FVB/N oocytes. Ten independent founder lines were generated. Line 619 was donated to the Jackson Laboratory and is representative of low expressing Apoe lines (lines 619 and 620). Only adrenal expression of Apoe was detected for these lines with Line 619 having the lowest mRNA expression (1.6 pg Apoe mRNA per microgram of total RNA). Mice have only 1-2% of wild-type plasma Apoe protein level. (J:130658)