Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis

The modified chicken beta-actin promoter (CAG) was used to drive ubiquitous expression of a floxed LacZ gene with a triple polyadenylation signal sequence followed by a constitutively active human ACVR1 sequence with the amino acid substitution of glutamine with aspartic acid at position 207 (Q207D), an IRES sequence, and the EGFP coding sequence and a poly(A) signal. Five lines were established and expression analysis for one line (line 35-1) confirmed general, but not ubiquitous, expression of LacZ and cre-dependent general expression of the modified ACVR1 and EGFP. (J:109498)