在exon 3内的编码区域被替换为lacZ基因和floxed neo cassette。通过E8.5至E9.5时期整个胚胎的 Western blot 分析,确认了没有蛋白质表达。(来源:J:142039)
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The coding region contained within exon 3 was replaced with a lacZ gene and floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on E8.5 to E9.5 whole embryo extracts. (J:142039)