Sell^tm2Tft

The membrane-proximal region was replaced with DNA encoding a floxed neomycin resistance gene and the 7 amino acid (APVVPTR) membrane-proximal region of mouse E-selectin. It was predicted that this mutation would prevent cleavage of the protein upon cellular activation. Cell surface levels of mutated protein in homozygote blood, spleen, and lymphocytes was 27%, 34%, and 52%, respectively, of wild-type controls as determined by flow cytometry. Mutated protein was not cleaved from the cell surface upon cellular activation of lymphocytes. (J:142184)