一段2.2kb的基因组DNA片段,包含所有ox外显子1,通过同源重组被neo cassette替换了。睾丸细胞匀浆的 Western blot 检测证实了纯合子中没有蛋白表达。(来源:J:141755)
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A 2.2 kb fragment of genomic DNA including all ox exon 1 was replaced with a neo cassette via homologous recombination. Western blot analysis of testicular cell lysates confirmed the absence of protein in homozygotes. (J:141755)