将连接在神经调质膜受体palmitoylation位点上的黄色荧光蛋白(YFP-Mem)插入目标基因的首编码区,由Runx3远端启动子驱动表达。在YFP的3'端,包含PGK-诺卡因抗性基因座,其两侧有loxP位点。在CD8+ T细胞和TH1亚型活化CD4+ T细胞中的目标基因蛋白表达几乎不可检测,且前者显著较低。YFP在MHC I限制的胸腺细胞、静息CD8+ T细胞以及活化CD4+ T细胞中表达。(来源:J:141144)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
--
9

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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