germ line(生殖细胞)中,通过Cre介导的重组手段移除了3到5号外显子。使用脑组织提取物进行 Western blot 分析,确实确认了小于正常量肽片段的降低。(来源:J:141236)
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Germ line, cre mediated recombination was used to remove exons 3 through 5. Reduced levels of a smaller than normal peptide were confirmed using western blot analysis on brain extracts. (J:141236)