从2号外显子到9号外显子的区域被替换为人类基因,其中3号外显子上游有一个attB53位点,9号外显子后面跟着一个由frt引导的neo cassette,末端有3'的attP50。最初使用的ES细胞带有Nr1i2tm1(NR1I2)Arte基因。通过germ线的flp介导重组,移除了neo cassette。通过在肝脏和小肠提取物中进行qPCR,确认了内源性转录物表达的缺失。(来源:J:140975)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
插入,基因内删除
--
1
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12

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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