Exons 2和3被替换成一个自我剪切的ACN-neo插件。在睾丸中,通过Cre介导的剪切发生了。RT-PCR和 Western blot分析证实了去雄性小鼠心脏中mRNA和蛋白表达的缺失。(来源:J:140286)
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Exons 2 and 3 were replaced with a self-excising ACN-neo cassette. Cre mediated excision occurred in the testes. RT-PCR and western blot analysis confirmed the absence of mRNA and protein expression in the hearts of hemizygous male mice. (J:140286)