Kras^tm5Tyj

This allele has a FRT-STOP-FRT cassette in intron 1 of the oncogenic form of the gene (p.G12D mutation). Before expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase, the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the STOP cassette is removed resulting in expression of the mutant Kras transcript and protein. (J:140469)