在exon 20的上游插入了loxP位点,下游则插入了loxP标记的诺卡因 cassette。通过基因组PCR确认了靶向的有效性。这个被修饰的exon带有polyadenylation信号,意味着当cre重组酶表达时,从这个位点产生的转录本会剪切掉,从而具有更短的半衰期。(来源:J:140205)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
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1
9
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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