Creb3l3^tm1.1Sad

A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. The floxed region included the neo cassette. Mice were crossed with mice carrying Tg(Ella-cre)C5379Lmgd to remove exons 4 through 11 and parts of introns 3 and 11. One loxP site remains downstream of exon 3. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombination events occurred. (J:140018)