Rr29^Rgsc1197

This mutation was identified in a screen of archived DNA from a large-scale ENU mutagenesis project for mutations in the cis-acting limb specific regulatory sequence ("mammal-fish-conserved-sequence 1", MFCS1) of the sonic hedgehog gene, which resides ~1 Mb proximal to Shh in intron 5 of the Lmbr1 gene. The mutation comprises an A-to-T mutation at position 1145 of this sequence; the nucleotide sequence flanking the mutation site is AACATACAAA [A-to-T] TAAATCTGAT. (J:133634)