Pkd1^tm3.1Jzh

A loxP-flanked neomycin selection cassette was inserted into the EcoRI site located 1.2 kb upstream of exon 2, and a third loxP site placed downstream of exon 6. Chimeric mice were crossed with Tg(Prm-cre)58Og transgenic mice that express cre recombinase in male germ cells. Offspring were selected that had exons 2 through 6 excised. RT-PCR analysis of kidney mRNA demonstrated a truncated mRNA where exon 1 splices to exon 8 resulting in a premature stop codon. Immunoblot assays on homozygote kidney extracts demonstrate no protein expression by this mutant allele. (J:140012)