整个编码区域被替换为一个带有floxed neo cassette的元件。通过在杂合子小鼠组织中进行实时PCR和 Western Blot分析,确实证实了转录和蛋白产量的减少。(来源:J:139044)
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The entire coding region was replaced with a floxed neo cassette. Reduction in transcript and protein production was confirmed by real-time PCR and western blot analysis on tissues from heterozygous mice. (J:139044)