在3号外显子和4、5号内含子,以及3号和4号 introns的位置插入了IRES-LacZ-neo cassette。通过同源重组,去除了3号和4号内含子。 Western blot结果显示,纯合胚胎中未检测到蛋白表达。(来源:J:139258)
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An IRES-LacZ-neo cassette was inserted into exon 3 and exons 4 and 5 and introns 3 and 4 were removed via homologous recombination. Western blot analysis confirmed absence of protein expression in homozygous embryos. (J:139258)