Exon 2, which contains the entire coding region of the gene, was replaced by targeting vector consisting of an EGFP/Cre-recombinase fusion gene followed by a floxed neomycin selection cassette. This targeting event generates a null allele. The EGFP/Cre-recombinase fusion product was detected in duct cells of the submandibular, sublingual and parotid glands. (J:139151)