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A targeting vector was prepared containing nucleotide mutations to produce a T50M mutation in the pre-ligand assembly domain of the gene. Mice were crossed with a Cre recombinase transgenic strain to remove a floxed neomycin selection cassette. Peripheral blood neutrophils from homozygotes have intracellular expression of the mutant protein but fail to express the protein on the cell surface. (J:139197)