在exon 1上游插入了loxP位点,然后在exon 1下游插入了可剔除的neo cassette。通过ES细胞中短暂的Cre表达,移除了neo cassette和exon 1。通过RT-PCR分析,对肝脏、肾脏和睾丸的提取物确认了转录表达的缺失。(来源:J:134843)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count