Hras^tm2Xbr

In ES cells containing Hras1tm1Xbr, a floxed hygro STOP cassette was inserted upstream of coding exon 1. In addition, a G to T substitutions was created at the second base of codon 12 of exon 1. This results in an amino acid substitution of valine for glycine. Germ line, cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12V mutation and the IRES-beta-geo cassette. (J:137729)