设计了一个靶向载体,包含一个由loxP切割位点引导的STOP cassette(后面跟着PGK-Neo-polyA和转录终止序列),位于绿色荧光蛋白/β-半乳糖苷酶融合蛋白序列(带有SV40核定位信号的GFP-NLS-lacZ或GNZ)的上游。这个完整构建通过电穿孔法插入到Gt(ROSA)26Sor位点。(来源:J:135149)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
--
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20

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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